Multispectral imaging of 7 organelles in live undifferentiated human induced pluripotent stem cells (hiPSCs).

Live undifferentiated KOLF2.1J human iPSCs were labelled with 7 organelle markers targeted to the nucleus, endoplasmic reticulum (ER), Golgi, mitochondria, peroxisomes, lysosomes, and lipid droplets. Complete z-stack and timelapse images were acquired in lambda mode (410-695 nm, 8.9 nm resolution) and subsequently linear unmixed into 8 different channels (channel 1 to 7 correspond to organelles, while channel 8 is the residual of the unmixing).