Changes in ROS production.

(A) PMA stimulated ROS production in PMNCs. Neutrophils were stimulated by 10 nmol/L PMA and ROS production was measured by Flow-cytometer as the intensity of DCFH fluorescence of 3000 cells. Bar graphs represent mean fluorescent intensity ± SD (*p<0.05 vs control with PMA stimulation, †p<0.05 vs AII with PMA stimulation, n = 6–8 for each group). (B–D) Superoxide production in the aortic wall. In situ detection of vascular superoxide production in Aorta (B), femoral arteries without cuff (C) and with cuff injury (D). Tissue superoxide was detected with DHE. Photomicrographs show representative results from 4 separate experiments. Scale bars indicate 50 µm in (B) and 500 µm in (C, D). Bar graphs represent mean fluorescent intensity ± SD (*p<0.05 vs Control, †p<0.05 vs BSO and AII, n = 7 of each). All statistical comparisons were evaluated using one-way ANOVA followed by Bonferroni post hoc analysis.