Sternaspis scutata µCT scan

µCT scan of a specimen of <em>Sternaspis scutata</em> stained with PTA for one week. Scan and reconstruction were obtained with the following settings: [System]<br> Scanner=SkyScan1272<br> Instrument S/N=15H09112<br> Software Version=1.1.10<br> Source Type=HAMAMATSU_L11871_20<br> Camera Type=XIMEA xiRAY16<br> Camera Pixel Size (µm)=7.4<br> Camera X/Y Ratio=1.0058 [Acquisition]<br> Number Of Files= 961<br> Number Of Rows= 1640<br> Number Of Columns= 2452<br> Partial Width=OFF<br> Image crop origin X=0<br> Image crop origin Y=0<br> Camera binning=2x2<br> Image Rotation=-0.00100<br> Optical Axis (line)= 890<br> Camera to Source (mm)=174.64085<br> Object to Source (mm)=82.59950<br> Source Voltage (kV)= 70<br> Source Current (µA)= 142<br> Image Pixel Size (µm)=6.999924<br> Scaled Image Pixel Size (µm)=6.999924<br> Image Format=TIFF<br> Depth (bits)=16<br> Reference Intensity=57000<br> Exposure (ms)=1238<br> Rotation Step (deg)=0.200<br> Use 360 Rotation=NO<br> Scanning position=11.719 mm<br> Frame Averaging=ON (4)<br> Random Movement=OFF (15)<br> Flat Field Correction=ON<br> FF updating interval=120<br> Geometrical Correction=ON<br> Filter=Al 0.5mm<br> Gantry direction=CC<br> Rotation Direction=CC<br> Type of Detector Motion=STEP AND SHOOT<br> Scanning Trajectory=ROUND<br> Number Of Horizontal Offset Positions=1<br> Number of connected scans=3<br> Current scan number=1<br> Number of lines to be reconstructed=1036<br> Scan duration=1h:40m:36s<br> Maximum vertical TS=5.0 [Reconstruction]<br> Reconstruction Program=NRecon<br> Program Version=Version: 1.7.1.0<br> Reconstruction engine=InstaRecon<br> Engine version=Version: 2.0.3.7<br> Reconstruction from batch=Yes<br> Postalignment=10.00<br> Connected Reconstruction (parts)=3<br> Sub-scan post alignment [0]=10.000000<br> Sub-scan post alignment [1]=10.000000<br> Sub-scan post alignment [2]=10.000000<br> Sub-scan scan length [0]=1035<br> Sub-scan scan length [1]=1035<br> Sub-scan scan length [2]=1036<br> Used extra shift in X per scan(micron)= 0.000 -1.993 -4.803 <br> Used extra shift in Y per scan(micron)= 0.000 -25.239 -44.091 <br> First Section=63<br> Last Section=3495<br> Reconstruction duration per slice (seconds)=0.349107<br> Total reconstruction time (2071 slices) in seconds=723.000000<br> Section to Section Step=1<br> Sections Count=3433<br> Result File Type=TIF<br> Result File Header Length (bytes)=12<br> Result Image Width (pixels)=2152<br> Result Image Height (pixels)=2056<br> Pixel Size (µm)=6.99992<br> Reconstruction Angular Range (deg)=192.20<br> Use 180+=OFF<br> Angular Step (deg)=0.2000<br> Smoothing=3<br> Smoothing kernel=2 (Gaussian)<br> Ring Artifact Correction=20<br> Draw Scales=OFF<br> Object Bigger than FOV=OFF<br> Reconstruction from ROI=ON<br> ROI Top (pixels)=2282<br> ROI Bottom (pixels)=223<br> ROI Left (pixels)=214<br> ROI Right (pixels)=2369<br> ROI reference length=2452<br> Filter cutoff relative to Nyquist frequency=100<br> Filter type=0<br> Filter type description=Hamming (Alpha=0.54)<br> Undersampling factor=1<br> Threshold for defect pixel mask (%)=0<br> Beam Hardening Correction (%)=54<br> CS Static Rotation (deg)=78.89<br> Minimum for CS to Image Conversion=0.003750<br> Maximum for CS to Image Conversion=0.105554<br> HU Calibration=OFF<br> BMP LUT=0<br> Cone-beam Angle Horiz.(deg)=11.863247<br> Cone-beam Angle Vert.(deg)=7.950323<br> Automatic matching in Z=50<br> Automatic matching in X/Y=50<br> Automatic matching in rotation=0.000000<br> Automatic fusion=1<br> <br> Data size has been reduced by conversion from 16-bit to 8-bit and decreasing the scale of the images.