Drug susceptibility of <em>Burkholderia pseudomallei</em> multidrug transporters assessed by an agar-based assay

The Gram-negative pathogen Burkholderia pseudomallei possesses multiple resistance-nodulation-division superfamily transporters that contribute to multidrug resistance, including BpeB and BpeF. Structural studies of BpeB and BpeF have identified a hydrophilic patch in their substrate-binding pocket. To investigate the relationship between this hydrophilic patch and substrate specificity, mutant analyses were performed using an Escherichia coli recombinant expression system. Drug susceptibility tests of BpeB and BpeF mutants showed up to a 64-fold increase in susceptibility compared with the wild type. Growth curve analyses revealed that BpeB mutants exhibited increased resistance to aminoglycosides, which are not transported by the wild type. These findings suggest that the hydrophilic patches in the substrate-binding pockets of BpeB and BpeF are involved in the substrate specificity. , , # Drug susceptibility of *Burkholderia pseudomallei* multidrug transporters assessed by an agar-based assay Dataset DOI: [10.5061/dryad.bnzs7h4qz](https://doi.org/10.5061/dryad.bnzs7h4qz) ## Dataset overview This dataset contains photographic images of agar plates obtained from drug susceptibility assays using *Escherichia coli* strains heterologously expressing multidrug transporters derived from *Burkholderia pseudomallei*. The images document bacterial growth in the presence of various drugs and compounds and were used to evaluate the contribution of the expressed transporters to drug susceptibility phenotypes. #### Experimental context Drug susceptibility agar plate assays were performed to assess the effects of *Burkholderia*-derived multidrug transporters on bacterial drug susceptibility. Recombinant *E. coli* strains expressing individual transporters were plated on agar media containing different drugs or compounds, and bacterial growth was documented by photography. These...,