Differential gene expression in SK-MEL-5 and MALME-3M cell lines following IFN-γ treatment

This study explores the effects of IFN-γ treatment on gene expression in two melanoma cell lines, SK-MEL-5 and MALME-3M. Using RNA sequencing, we aimed to identify differentially expressed lncRNA genes that respond to IFN-gamma, which could illuminate pathways involved in immune response modulation in melanoma cells. We conducted an RNA sequencing experiment on both cell lines, both treated and non-treated with IFN-γ. Cells were seeded in 6-well plates in triplicates, and treatment was initiated at the time of seeding. IFN-gamma was administered for 24 hours, after which RNA was extracted using the miniRNAeasy kit (Qiagen, Cat# 217604) according to manufacturer’s instructions. Library preparation followed and RNAseq was performed with NovaSeq SP 200 kit (2 x 100 cycles).