Development and proof of concept evaluation for a low-resource compatible Chikungunya virus diagnostic

Chikungunya virus (CHIKV) is a positive sense RNA Alphavirus that continues to pose major public health threats throughout the world. CHIKV is primarily transmitted via the Aedes genus mosquito; however, it has also exhibited transmission routes via blood transfusion and vertical transmission (mother to child). With only one approved vaccine thus far and no approved medicines, early detection is crucial in mitigating CHIKV outbreaks. Here, we designed and evaluated a sensitive and specific CHIKV diagnostic using reverse transcription-recombinase aided amplification (RT-RAA) coupled lateral flow strip detection (LFD) targeting a highly conserved region of the CHIKV E1 gene. Our results demonstrate that using our simple sample preparation reagent (TNA-Cifer-E) can inactivate live CHIKV in two minutes at room temperature, whilst also sustaining viable viral RNA. Our specificity analysis demonstrates that the Iso-CHIKV-Dx does not detect any closely related Alphaviruses nor any of the commo..., , # Data from: Development and proof of concept evaluation for a low-resource compatible Chikungunya virus diagnostic

Dataset DOI: [10.5061/dryad.547d7wmmf](10.5061/dryad.547d7wmmf)

## Description of the data and file structure

These data were generated to develop, optimize, and validate a proof-of-concept rapid, isothermal CHIKV test using reverse transcription recombinase aided amplification coupled with lateral flow detection (RT-RAA/LFD) and a rapid sample preparation for low-resource virus detection in Urine.

### Files and variables

#### File: Raw_Data_for_Figure_1.fasta

**Description:** Raw, aligned sequence data of 667 unique CHIKV E1 gene sequences along with a consensus sequence.

#### File: Raw_Data_for_Figure_2_.csv

**Description:** Limit of detection calculation of CHIKV RNA copies per micro litre using RT-RAA-LFD.

##### Variables: Viral RNA copies/uL, Pixel density (black and white normalised values)

* Raw image file: Uploaded separately as a PNG file. Depicting raw ...,