Exp_20220122_1_BT_RI_batch_WC_medium_2

Growth kinetics of monocultures Bacteroides thetaiotaomicron VPI-5482 (DSM 2079T) and Roseburia intestinalis L1-82 (DSM 14610T) and the co-culture in Wilkins-Chalgren Anaerobe Broth Notes: live/dead cell staining followed by flow cytometry, using a benchtop CytoFLEX S flow cytometer (Beckman Coulter, Brea, USA); anaerobic cultivation of 120 h for the experiment 2 In addition to the instrumental calibration with CytoFLEX Daily QC Fluorospheres, 0.5 μm and 1 μm green-fluorescent beads (Thermo Fisher Scientific, USA) were applied to each batch of experiments as internal standards, to monitor the instrument stability and to make cytometric data from different batches more comparable.