Transcriptomic analysis of the effect of Sfp1 depletion

The effect of Sfp1 depeltion at transcriptional level was investigated by construting a AID-Sfp1 strain (Auxin sensitive degron) that allowed to shutoff the Sfp1 expression and analyze the primary effects on synthesis rates (SR) and mRNA levels (RA) genome-wide. mRNA stabilities can be calculated as RA /SR for each gene in all samples. For the degron experiment, the auxin degron strain (AID-Sfp1) was inoculated in YPD medium until a OD600 of 0.4 and then an aliquot was taken, corresponding to t=0. After auxin addition two more samples were taken at 20 and 60 minutes. At each time point (0, 20 and 60 minutes) Genomic Run-on (GRO) was performed, as described in (Oliete-Calvo et al., 2018), and data for SR, RA were obtained for each time point. Note that each sample record represents two (cDNA) and (GRO) replicates .