Fourteen isolates and six ulcer specimens from tularemia patients in Sweden 2008 characterized by the developed hierarchical real-time PCR array.

aStrain ID in the Francisella Strain Collection, Swedish Defense Research Agency, Umeå, Sweden.
bSample ID at the Department of Clinical Bacteriology, Umeå University, Umeå, Sweden.
cIsolate FSC777 and ulcer specimens 32–38 are from the same patient. Isolate FSC778 and ulcer specimens 32–47 are from the same patient.
dThe exact genotype could not be determined due to detection failure of marker B.22 (the difference in time of appearance between the two PCR products was less than one cycle).
eF. tularensis cultures were negative.
fThe patient reported probable acquisition of tularemia when visiting the county of Jämtland, where the regional center is Östersund.
gThe genotype and subclade were assigned based on marker B.20, which exhibited an A for all three specimens, and on marker B.23, which exhibited a T. No other markers were screened due to scarcity of DNA.
hThe genotype and subclade were assigned based on: marker B.20, which exhibited a G for all three specimens; on marker B.21, which exhibited a G for specimens 32–215, and an A for specimens 32–38 and 32–47; and on marker B.22, which exhibited a G for specimens 32–38 and 32–47. No other markers were screened due to scarcity of DNA.