Optimization of mass cytometry sample cryopreservation after staining

This report describes a strategy to cryopreserve stained cells in hope of alleviating numerous technical limitations with current sample-handling approaches. Here we evaluated two protocols of freezing at -80oC in 10% DMSO in FBS and 100% methanol against standard refrigeration at 4oC in staining buffer. Conclusion: We conclude that freezing already stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve samples when needed. EQ Four Element Calibration Beads (Fluidigm Corporation, South San Francisco, CA) were added at a concentration of 1% prior to acquisition.