Comparison of the assay results with the known redox mechanisms of 5-LO inhibitors.

The results of two redox assays and the known redox mechanisms of tested compounds were compared. The lower EC50 value from fluorescence assay signifies the stronger redox activity. When the calculated EC50 was over the experimental range, it was indicated as having >100 µM of EC50. The change in absorbance (Δ abs) of each inhibitor was calculated by subtracting the absorbance at 180 seconds from the starting absorbance value. The tests were performed in triplicate, and their averages and standard deviations are presented. The known mechanisms are explained in the materials and methods section.
The redox fluorescence assay correctly distinguished the three non-redox inhibitors by having high EC50 values. Meanwhile, the redox absorbance assay predicted contradictory results to known mechanism for several redox compounds, such as NDGA and CDC.