Transcriptomic analysis of mice treated with Lactobacillus rhamnosus CNCM i-3690 and different mutants and variants generated from this strain

we have performed transcriptomic analysis of colonic samples of mice challenged with DNBS twice in a chronic model of inflammation and treated with CNCM -I3690 strain and several mutants and variants generated from it. colonic transcriptome analysis at the endpoint revealed that CNCM I 3690 enhances the expression of genes related to healthy gut permeability, motility (Ghrelin (GHR)), and absorption (guanylate cyclase activator 2B (Guca 2b)); cell proliferation (amino acid transporter SLC7A7); and protective functions (endogenous protease inhibitor kazal-type4). Focusing on inflammation, both GHR and Guca 2B have been found to act as anti-inflammatory, protecting the gut against a wide range of threats. Besides, CNCM I-3690 also up regulated TRAF-interaction protein with a forkhead-associated domain (TIFA), an important signaling adaptor in the NF-κβ pathway. To further analyze the mechanisms of action of CNCM I-3690 in a murine model of gut inflammation we have performed transcriptomic analysis of colonic samples of mice challenged with DNBS and treated with this strain and several mutants and variants generated from it