File S1 - Identification of Unprecedented Anticancer Properties of High Molecular Weight Biomacromolecular Complex Containing Bovine Lactoferrin (HMW-bLf)

Figures S1–S4. Figure S1. Representative microscopy images showing trypan blue exclusion assay when the cells were grown in their respective growth media with 1% FBS for 24 h, indicating the >98% viability. This indicates that serum deprivation on incubation with bLf treatments for cellular uptake in 1% FBS containing assay media does not compromise the ability of the cells to exclude the dye and they remained healthy with intact membranes for cellular uptake of bLf. Magnifications 40X. Figure S2. Representative graph of the percentage iron content in the different forms of bLf. Figure S3. High resolution graph of FTIR spectra. The Fe-O vibration band appears at 560 cm-1 in the FTIR spectrum of Fe-bLf, and it is not pronounced in the other three spectra suggesting the high iron content in Fe-bLf and confirming iron content estimation. Figure S4. Cell death (mortality count) in FHs 74 Int as measured by Flow cytometry using propidium iodide staining (* p (DOCX)