Metabolomic and transcriptomic profiling of adult mice and larval zebrafish leptin mutants reveal a common pattern of changes in metabolites and signaling pathways [Dataset 2]

Leptin plays a critical role in the regulation of metabolic homeostasis. However, the molecular mechanism and cross talks between leptin and metabolic pathways leading to metabolic homeostasis across different species is not clear. This study aims to explore the effects of leptin in mice and zebrafish larvae by integration of metabolomics and transcriptomics. We performed gene expression profiling analysis using data obtained from RNA-seq. . Deep sequencing showed that many genes involved in proteolysis and arachidonic acid metabolism were dysregulated in ob/ob mice heads and lepb mutant zebrafish larvae compared to their wild type counterparts, respectively. Male ob/ob mice and lean C57BL/6 wild type mice were obtained from Charles River Laboratories at 6 weeks of age (n=8 per group) and maintained for 8 weeks under specific pathogen-free conditions in the animal facility of the Leiden University Medical Center (LUMC). Heads were used to exctract RNA for RNAseq.瘦素（Leptin）在代谢稳态（metabolic homeostasis）的调控中发挥关键作用。然而，不同物种间介导代谢稳态的瘦素与代谢通路之间的分子机制及其串扰（cross talks）仍未明确。本研究拟通过整合代谢组学（metabolomics）与转录组学（transcriptomics）技术，探究瘦素在小鼠及斑马鱼幼体中的生物学效应。本研究利用RNA测序（RNA-seq）获取的数据集开展了基因表达谱分析。深度测序结果表明，相较于各自的野生型对照，ob/ob小鼠头部中诸多参与蛋白水解（proteolysis）的基因出现表达失调，而lepb突变型斑马鱼幼体中诸多参与花生四烯酸代谢（arachidonic acid metabolism）的基因亦存在表达异常。本研究于6周龄时从查尔斯河实验室（Charles River Laboratories）购入雄性ob/ob小鼠与健康C57BL/6野生型小鼠（每组n=8），并在莱顿大学医学中心（Leiden University Medical Center, LUMC）动物设施的无特定病原体（specific pathogen-free, SPF）饲养条件下常规饲养8周。随后摘取小鼠头部组织，用于提取RNA以开展RNA测序（RNA-seq）。