Identification of bicyclomycin-sensitive transcripts in Vibrio cholerae. Identification of bicyclomycin-sensitive transcripts in Vibrio cholerae

In this study we used BCM, inhibitor of Rho activity, to determine Rho-dependent transcription termination sites in V. cholerae. Overall design: V. cholerae oppA::3xFLAG oppB::3xFLAG strains with wild-type oppB or the oppB start codon mutated to ATC were grown to early stationary phase (OD600 of 1.5). Cultures were divided in half and treated with either H2O (mock treatment) or BCM (25 µg/ml final conc.) for 2h before RNA samples were collected. The experiment was performed with three independent biological replicates.