Evaluation of the inflammatory and osteogenic response induced by metal particles released during implantoplasty of dental implants. Evaluation of the inflammatory and osteogenic response induced by metal particles released during implantoplasty of dental implants

During implantoplasty there is a large release of metal particles that might induce a pro-inflammatory response and reduce the viability of osteogenic cells, which may compromise the results of dental implants The analysis of the inflammatory response in macrophage cell culture (THP-1) was carried out by RT-qPCR. CCR7, TNF-α and IL-1β genes were analyzed as proinflammatory markers, while CD206, TGF-β and IL-10 were analyzed as antiinflammatory markers. The osteogenic response was studied with human bone marrow mesenchymal stem cells (BM-MSCs). We analyzed Runx2, alkaline phosphatase (ALP) and osteocalcin (OC) genes. Overall design: In vitro cell based assay study. The expression of genes was analysed by means of RT-qPCR. We used THP-1 and BM-MSCs cells to study the inflammatory and osteogenic response, respectively.