Reptile_transcriptomics. Reptile_transcriptomics

High-throughput sequencing of RNA from secretory tissues of reptiles. Tissues were dissected from freshly-euthanised individuals and either snap-frozenin liquid nitrogen or stored short-term in RNAlater (Ambion). Total RNA was extracted from approximately 30mg of tissue using the Qiagen RNeasy Mini kit according to manufacturer instructions, with on-column DNase treatment. Typically, single whole scent glands and venom/salivary glands were used in the relevant extractions and skin samples were taken from the dorsal side at approximately mid-body level. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/