Differentially expressed Proteins in ERU detected by 2D-DIGE and identified by mass spectrometry.

Differentially expressed proteins in lymphocytes of spontaneous ERU cases. Spots were excised from silver stained 2D-DIGE gels and identified by MALDI-TOF/TOF mass spectrometry; proteins listed were identified with a probability score that was significant with p<0.05. (a) protein number as shown in figure 1, (b) protein name and (c) accession number as listed in Ensembl horse protein database (www.ensembl.org), (d) theoretical molecular weight and (e) theoretical isoelectric point of respective protein, (f) probability based MOWSE score; score is -10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 60 were significant (p<0.05), (g) peptide count from MALDI-TOF/TOF analysis. Differential protein abundance was detected by DeCyder 6.5 software, providing (h) the appearance of each protein spot among the DeCyder map images of the experiment (number in parentheses: three images were generated per gel: control, ERU and internal standard, adding up to a maximum of 15 possible spot maps), (i) fold change (>1.5) and (k) the p-value for differential expression of proteins comparing healthy state and ERU cases (Student's t test). (l) Expression in ERU specimen was compared to controls.