Tests for neutrality for each gene.

Tajima's D, and Fu and Li's D* and F* were calculated using the online Intrapop Neutrality Test calculator (http://wwwabi.snv.jussieu.fr/achaz/neutralitytest.html, [38]). N = # samples; S = # segregating sites, i.e. polymorphic nucleotides; θπ = #mutations (theta) per site from pi (nucleotide diversity); and θS = theta per segregation site. Bold P-values indicate statistical significance based on 100,000 replicates with no recombination, and normalized by their standard deviations. Significant positive D/D*/F* indicates balancing selection or bottleneck; significant negative D/D*/F* indicates directional or expansion.