Data_Sheet_1_Enhancement of the Stability and Anti-DPPIV Activity of Hempseed Hydrolysates Through Self-Assembling Peptide-Based Hydrogels.docx

Although there is an increasing interest for bioactive food protein hydrolysates as valuable ingredients for functional food and dietary supplement formulations, their potential applications are hampered by their insufficient stability in physiological conditions. In this study, an innovative strategy based on nanomaterials was developed in order to increase the hempseed hydrolysate stability and the anti-diabetic properties, through their encapsulation into ionic self-complementary RADA16 peptide based-hydrogels. Atomic force microscope (AFM) morphological analysis indicated that the new nanomaterials were composed of a nanofibril network, whose increased diameter in respect to native RADA16 suggests the presence of transient non-covalent interactions among the RADA16 supramolecular assemblies and the embedded hempseed peptides. Structural analysis by FT-IR spectroscopy indicated typical β-sheet signatures. The RADA16-hempseed protein hydrolysate hydrogel was shown to act as a novel dipeptidyl peptidase IV (DPPIV) inhibitor in different biological assays. Finally, this nanoformulation was used as a drug delivery system of the anti-diabetic drug sitagliptin, helping to reduce its dosage and eventually associated side-effects.

尽管对生物活性食品蛋白水解物作为功能性食品和膳食补充剂配方中宝贵成分的兴趣日益增长，但其潜在应用受到其在生理条件下的稳定性不足的制约。本研究开发了一种基于纳米材料的新型策略，旨在通过将大麻籽水解物封装于基于离子自互补RADA16肽的亲水凝胶中，以增强其稳定性和抗糖尿病特性。原子力显微镜（AFM）形貌分析表明，新的纳米材料由纳米纤维网络组成，与原生RADA16相比，其直径的增加暗示了RADA16超分子组装体与嵌入的大麻籽肽之间存在瞬时的非共价相互作用。傅里叶变换红外光谱（FT-IR）结构分析揭示了典型的β-折叠片层特征。RADA16-大麻籽蛋白水解物亲水凝胶在多种生物实验中被证明是一种新型二肽基肽酶IV（DPPIV）抑制剂。最终，这种纳米制剂被用作抗糖尿病药物西格列汀的药物递送系统，有助于降低其剂量并最终减少相关副作用。