Identification of Sam68-PxxP-motifs responsible for SH3-domain-binding.
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Peptides corresponding to the seven PxxP-motifs (P0 to P6) of Sam68 were purified as GST-fusions and analyzed for interaction with the indicated SH3 domains by phage-ELISA. Results are expressed semi-quantitatively as half-maximal binding occuring at <1011 (+++), 1011−1012 (++), >1012 cfu/ml (+).
创建时间：
2012-06-20
