The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis [ChIRP-seq]

We identified differentially expressed genes in flail mutants linked to the regulation of flowering through stranded RNA-seq. A genomic rescue fragment of FLAIL (pFLAIL:gFLAIL) introduced in flail mutants can complement expression defects and early flowering, consistent with trans-acting effects of the FLAILRNA. We determined the genomic binding regions of FLAIL by ChIRP-seq. FLAIL binding to chromatin regions promotes the expression of selected flowering repressors. ChIRP-seq was used to determine chromatin binding regions of FLAIL.