Gene expression of liver sinusoidal endothelial cells from an 8-week-old WT and ob/ob mouse

Obesity may promote myeloid cell accumulation in liver. Intravital liver tissue imaging revealed that the number of myeloid cells attached to sinusoidal wall significantly increased in ob/ob mice. To assess the pathophysiological roll of liver sinusoidal endothelial cells (LSEC) in obese livers, we performed microarray analysis in LSEC isolated from an 8-week-old wild-type and ob/ob mouse. Pathway analysis in microarray data shows genes related to cell adhesion, chemokine signaling and leukocyte transendothelial migration are upregulated in LSEC from ob/ob. Liver sinusoidal endothelial cells were isolated by collagenage digestion of livers from an 8-week-old male wild-type and ob/ob mouse. Cells were cultured overnight in DMEM containing 10% FBS.