Time-restricted feeding mediated transcriptomic changes in the indirect flight muscle tissue in Drosophila obesity models

The goal of the study was to investigate the mechanistic basis for Time-restricted feeding (TRF) improvement in skeletal muscle by assessing transcriptomic data of wild type (WT), WT under High-fat diet (HFD), and genetic obesity linked mutant sphingosine kinase 2 (Sk2) under ad libitum feeding (ALF) and time-restricted feeding (TRF) conditions. Next generation sequencing was used to assess the changes along a diurnal cycle in the transcriptome of Drosophila indirect flight muscle (IFM) tissue at 3-week of age under ad libitum feeding (ALF) or time-restricted feeding (TRF). Overall design: After 3 weeks of age on the assigned diet (normal and HFD) and feeding regimen (ALF & TRF), indirect flight muscle (IFM) was collected from 10 female flies every 4 h over 24 h and samples were flash frozen. Total RNA were prepared using the RNeasy kit (QIAGEN). Libraries were prepared according to Illumina protocols for single-end 50 bp mRNA sequencing and sequenced on an Illumina machine. Reads were aligned to the Drosophila melanogaster genome (BDGP r5) using STAR (v2.3.0e).