Nano LC-MS/MS analysis of purified GST-CK1δ305–428.
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Phosphate-labeled GST-CK1δ305–428 was isolated and digested by trypsin. The resulting phosphopeptides were analyzed by Nano LC-MS/MS analysis as described in Materials and Methods. Mass spectra were analyzed using the software suite MaxQuant, version 1.0.14.3. Potential phosphorylated peptides and residues matching the sequence of rat CK1δ are shown. Ph, phosphorylation; ox, oxidized.
创建时间:
2015-12-02



