Effect of depletion of Pcyt2 on gene expression in the early myotome.

To investigate the cosequence of Pcyt2 deletion in the early myotome, we established Myf5Cre-Pcyt2 mouse line in which each target gene has been depleted in the early developing myotome and onwards in the mature muscle. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points. Comparative gene expression profiling analysis of RNA-seq data for developing muscle in control (Pcyt2 flox/flox) mice and Myf5Cre-Pcyt2 experimental group on the same background with muslces defficient for Pcyt2..