A single-cell RNA sequencing atlas of circulating leukocytes from healthy and osteosarcoma affected dogs

We used single-cell RNA sequencing to characterize the heterogeneity of circulating leukocytes in dogs, then employed the dataset to investigate how primary osteosarcoma (OS) tumors impacted circulating leukocytes. The complete dataset consists of circulating leukocytes from 7 healthy dogs and 10 OS affected dogs. The final processed Seurat objects are provided as 'final_dataSet_H.rds' (Healthy only) and 'final_dataSet_HvO.rds' (Healthy and osteosarcoma PBMCs). Each major immune cell type subset is provided where 'cytotoxic.rds' = CD8/NK cell, 'helper.rds' = CD4 T cells, 'myeloid.rds' = Myeloid, and 'bcell.rds' = B cells. All R code used to complete the analysis can be found at: https://github.com/dyammons/Canine_Leukocyte_scRNA.