Systemic sensitization with ovalbumin (OVA) induced mild allergic dermatitis when compared to additional topical OVA applications.

e.c., epicutaneous; i.p., intraperitoneal; OVA, ovalbumin; PBS, phosphate-buffered saline; Th, T-helper cell.Data are indicated as mean ± SEM. Statistical significance (p) was tested using one-way ANOVA followed by Tukey’s multiple comparison test.*p**p***p#p##p###p1Positively stained cells were counted in six fields per section of n = 5 (IHC-staining) or n = 8 animals (Giemsa-staining), respectively, and expressed as cells per mm2.2Determined in Giemsa-stained skin sections.3Determined in IHC-stained skin sections.4Cytokine levels in sera were determined as triplicate measurements of n = 4 mice per group using Quantikine mouse immunoassay kits. Data are indicated as pg/ml.