Histone modification profiling (using the iChIP protocol) for two markers of regulatory activity (H3K4me3, H3K27ac) from a panel of genetically diverse crosses of Drosophila melanogaster during embryogenesis

Profiling of histone modifications with the iChIP protocol (PMID25103404) using antibodies against two markers of regulatory activity: H3K27ac (abcam, ab4729) and H3K4me3 (Millipore, 07-473). All paternal fly lines were taken from the Drosophila Genetic Reference Panel crossed to a common mother (PMID31308546). Data were collected at three time points (2-4h, 6-8h, 10-12h at 25C) with two biological replicates per collection.