Single-cell RNA sequencing data of Ly49? CD8 T cells treated with anti-CD80/86 antibody and control Ig

This study aims to investigate the functional role of Ly49+CD8+T cells in allogeneic immune responses during cardiac transplantation. Using a murine heart transplant model and mixed lymphocyte reactions, we analyzed how these cells respond under CD80,86-CD28 co-stimulation blockade. The results revealed that Ly49+CD8+T cells exhibit distinct proliferative and cytotoxic features and contribute to immune regulation through Qa-1 interaction and inhibitory receptors such as LAG-3 and CTLA-4. This research provides new insights into the immunoregulatory mechanisms that promote graft tolerance and may inform future strategies to prevent transplant rejection.