Subtype specific analyses reveal Infiltrative Basal Cell Carcinoma are highly interactive with their environment

Little is known regarding the differences between BCC subtypes, despite clearly distinct phenotypes and clinical outcomes. In particular, what distinguishes the aggressive infiltrative BCC subtype. We determine that infiltrative BCC do not contain an obviously distinct genomic variant profile however show clear molecular signalling differences compared to less aggressive subtypes. RNA sequencing enabled identification of candidate regulators of infiltrative subtype development such as Wnt and Integrin signalling, and suggested that infiltrative BCC have a strong relationship with their surrounding environment. In particular, WISP1 and Periostin gene expression is associated with the infiltrating subtype and represent candidate regulators of subtype differences in human BCC. RNA extraction direct from human tissue used to generate RNA profiles for BCC subtype. BCC subtype indicates majority subtype, classified via histological investigation.