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GRO analysis of topoisomerase mutants

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16673
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Genomic Run-on analysis of topoisomerase mutants (top1-delta/top2ts or top2ts) has been conducted at reference temperature for wt strain and at non-permissive temperature for wt and mutant strains. Keywords: Genomic Run On The analysis includes 3 repeats of the wild type strain (JCW25) at 30ºC and 3 more at 37ºC, 3 repeats of the top2ts mutant (JCW26) at 37ºC and 3 repeats of the double top1 (delta) top2ts mutant at 37ºC. A single genomic DNA hybridization on one of the filters can be used for normalization between gene probes if needed.
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2017-05-09
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