Antigen-induced IFN-γ secretion by PBMCs from 30 M. bovis BCG-vaccinated healthy subjects and ProPred predictions for PPE68 and its peptides (P1 to P24) to bind 51 HLA-DR alleles.

aIFN-γ responses were evaluated by stimulating PBMCs with the peptides of PPE68 according to procedures described in materials and methods. The strong responses (Median concentration >5 U/ml and %positive≥70%) are given in bold face.bHLA-DR binding predictions for complete PPE68 sequence and its individual peptides were analyzed using the ProPred server (http://www.imtech.res.in/raghava/propred/). The % binding values suggesting promiscuous HLA-DR binding (binding to >50% HLA-DR alleles) are shown in bold face.P/T = Number of subjects positive/Number of subjects tested.