Next Generation Sequencing Quantitative Analysis of soybean seeds Transcriptomes. Next Generation Sequencing Quantitative Analysis of soybean seeds Transcriptomes

To investigate the regulatory mechanisms of GmNFYA in salt stress, leaves and roots of transgenic plants and JACK plants were collected for RNA extraction and sequencing. Clean reads of RNA-sequencing were mapped to soybean genome and genes that had a 2 fold increase or 50% decrease, compared to JACK, were defined as differential expressed genes (DEGs). Overall design: mRNA profiles of soybean leaf and roots. Wild type (Jack) and GmNFYA overexpressing soybean.