Circulating lncRNAs NONHSAT054669.2 and ENST00000525337 can be used as early biomarkers of gestational diabetes mellitus

This study aimed to identify key circulating long non-coding RNAs (lncRNAs) as novel biomarkers for diagnosis of GDM at the early stages. First, lncRNA microarray analysis was conducted for plasma samples of GDM women before delivery and 48 h after delivery. The expression of differentially expressed lncRNAs in clinical samples at different trimesters was randomly validated by quantitative PCR. Moreover, the correlation between lncRNA expression and oral glucose tolerance test (OGTT) level in GDM women during the second trimester was analyzed, and the diagnostic value of key lncRNAs for GDM during different trimesters was evaluated by receiver operating characteristic (ROC) curve. The plasma level of NONHSAT054669.2 and ENST00000525337 may be used as novel diagnostic biomarkers for early diagnosis of GDM. We performed lncRNA microarray analysis of plasma samples from 3 pregnant women with GDM before delivery and 48 hours after delivery to identify key lncrnas associated with GDM