RNA-Seq analysis of 9G8-RNAi fat bodies in Drosophila

Previous studies from our lab have characterized a group of splicing factors called SR proteins that function to identify intron/exon borders in regulating metabolic homeostasis in the Drosophila fat body. Decreasing the function of one SR protein, 9G8, causes an increase in triglyceride storage; however, the full complement of 9G8 target genes that regulate metabolism is unknown. To address this question, we performed RNA sequencing on Drosophila fat bodies with 9G8 levels reduced by RNAi. Drosophila fat body mRNA proflies of yolk-Gal4>9G8-RNAi fat bodies compared to yolk-Gal4/+ control fat bodies (4 samples of each genotype).