mRNA transcript distribution bias between Borrelia burgdorferi bacteria and their outer membrane vesicles

We performed comparative transcriptomic analysis of the outer membrane vesicles (OMVs) released from B. burgdorferi. We identified a total of ~1200 unique transcripts with at least one mapped read from the bacterial cell and its OMVs. Sequencing of ribosomal depleted Borrelia burgdorferi strain B31 and its OMVs isolated using two methods, ultracentrifugation and size-exclusion chromatography