IL-4/STAT6 signaling in eosinophils is required for larval growth.

(A) Body size (area) of larvae, 17dpi. ΔdblGATA mice received PBS or 5 × 106 eosinophils every 48 h between 5 and 9 dpi or 11 and 15 dpi (oral infection). (B)–(C), ΔdblGATA mice received PBS or 5 × 106 eosinophils from infected IL-5Tg+ or IL-5Tg/STAT6-/- mice every 48 h from 5–9 dpi. (B) Total body larval burdens in muscle, 28 dpi. (C) Body size (area) of larvae, 17 dpi. (D) ΔdblGATA mice received PBS or 5 × 106 eosinophils from infected IL-5Tg+ or IL-5Tg+/IL-4-/- mice every 48 h from 5–9 dpi. Body size (area) of larvae, 17dpi. (E) ΔdblGATA mice received PBS or 5 × 106 eosinophils from uninfected or infected IL-5Tg+ every 48 h from 5–9 dpi. Body size (area) of larvae, 17dpi. (F) Body size (area) of larvae recovered from C57BL/6 and IL-5-/- mice injected 25,000 NBL IV, 13 days post injection. (G) IL-4-/- mice received PBS or 5 × 106 eosinophils from infected IL-5Tg+ or IL-5Tg+/IL-4-/- mice every 48 h from 0–5 days post IV infection. Body size (area) of larvae, 13 days post injection. Each data set was collected from two experiments with similar results. Values represent mean ± SD; n = 4 mice. Significant differences were determined by ANOVA and Tukey’s test. *p < 0.05, **p < 0.001, ***p < 0.0001.