Production of extra DTCs in mutants of cell-cycle regulators.

The extra-gonad phenotype was scored under Nomarski optics. The extra-DTC phonotype was scored in strains carrying lag-2::GFP. In dpy-5 ar95 animals, the extra-gonad phenotype was observed less frequently than the extra-DTC phenotype, because the shape of the gonads was difficult to observe in the dpy-5 background, especially for gonads on the far side of the animal from the objective lens. Heat shock was applied at the early (0–5 hrs after hatching), middle (7–11 hrs after hatching), or late (13–15 hrs after hatching) L1 stage for 2 hrs at 33°C. The percentage of animals with extra gonads or DTCs is shown. n: number of animals scored. N. D.; not determined. a Compared with wild type. b Compared with cye-1(os66) mutants. c Compared with cdk-2(RNAi) animals. d Compared with wild type with heat shock at middle L1. See text.