Transcriptional portrait of M. bovis BCG during in vitro biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment.. Transcriptional portrait of M. bovis BCG during in vitro biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment.

By RNA-seq analysis, we monitored the early steps of biofilm production in BCG, to distinguish intercellular aggregation from attachment to a surface, and found a number of genes being differentially expressed at these stages. Overall design: We analyzed samples of BCG grown in Sauton medium, at 24 h, 7 days, and 10 days postinoculation in 25cm2 flasks, individually. The experiment was repeated once, so we had a total of 6 samples analyzed by RNASeq.