Gene annotation was primarily performed by using KofamKOALA. Unannotated genes would be aligned with Uniprot database by DIAMOND. OGs were determined by eggnog-mapper. Raw reads were first tirmmed by
Melt-curve plots.pdf : qPCR melt curve plots of SRPK2 and CHDH generated using the QuantStudio 3 Real-time PCR software. The melt curves are generated by plotting Derivative Reporter (-Rn′) vers
A search for DUET expressologs (identical proteins with similar expression pattern) in Poplar, Medicago, Soybean, Rice, Barley, Maize, Potato and Tomato returned the proteins listed above (http://bar.
