Genome-wide map of RNA polymerase II enrichment in Wild-Type and Ipa1-1 mutant samples

We report on a comparison of ChIP-seq comparison of wild-type (BY4741) and Ipa1-1 mutant yeast, using duplicate samples of each genotype. Our analysis shows that loss of Ipa1 function leads to loss of 3'-end formation, and specifically shows a distinct gain of occupancy immediately downstream of polyA sites, suggesting that inefficient cleavage is causing PolII to remain attached ot the genomic DNA for relatively longer periods of time. Examination of Ipa1-1 mutation induced changes in polII occupancy