Primary transcriptome and translatome analysis determines transcriptional and translational regulatory elements encoded in the Streptomyces clavuligerus genome. Primary transcriptome and translatome analysis determines transcriptional and translational regulatory elements encoded in the Streptomyces clavuligerus genome

We obtained the high-quality genome sequence of S. clavuligerus ATCC 27064, and determined genome-wide TSSs. Then, RNA-Seq and ribosome profiling were additionally exploited to reveal fundamental regulatory elements for transcription and translation. Overall design: Transcriptome (RNA-seq), translatome (ribosome profiling), primary transcriptome (dRNA-seq, pooled) data of Streptomyces clavuligerus ATCC 27064 at four different growth phases in duplicates using Illumina HiSeq本研究获取了克拉维酸链霉菌（Streptomyces clavuligerus）ATCC 27064的高质量基因组序列，并鉴定了全基因组范围的转录起始位点（Transcription Start Sites, TSS）。随后，本研究额外采用RNA测序（RNA-Seq）与核糖体谱分析（ribosome profiling）技术，解析转录与翻译过程的核心调控元件。实验设计概述：使用Illumina HiSeq测序平台，对克拉维酸链霉菌ATCC 27064的四个不同生长阶段各设置两次生物学重复，获取其转录组（RNA-seq）、翻译组（ribosome profiling）及初级转录组（dRNA-seq，混合样本）数据。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于链霉菌（Streptomyces clavuligerus）ATCC 27064的初级转录组和翻译组分析，旨在通过全基因组转录起始位点测定、RNA-Seq和核糖体分析，揭示其转录和翻译的调控元件。数据覆盖了四个不同生长阶段的重复实验，属于多分离物范围的转录组或基因表达数据，为理解链霉菌的基因调控机制提供了关键资源。