Weights of hatchlings across different temperature treatments

Global warming is one of the most significant and widespread effects of climate change. While early life stages are particularly vulnerable to increasing temperatures, little is known about the molecular processes that underpin their capacity to adapt to temperature change during early development. Using a quantitative proteomics approach, we investigated the effects of thermal stress on octopus embryos. We exposed Octopus berrima embryos to different temperature treatments (control 19 °C, current summer temperature 22 °C, or future projected summer temperature 25 °C) until hatching. By comparing their protein expression levels, we found that future projected temperatures significantly reduced levels of key eye proteins such as S-crystallin and retinol dehydrogenase 12, suggesting the embryonic octopuses had impaired vision at elevated temperature. We also found that this was coupled with a cellular stress response that included a significant elevation of proteins involved in molecular ..., Female Octopus berrima (Stranks & Norman 1992) (n = 9) were obtained in October 2021 (austral spring) from an artisanal octopus fishery at Venus Bay, South Australia using unbaited octopus pots. Unlike other merobenthic octopus species that produce small planktonic hatchlings, Octopus berrima is a holobenthic species that produce large, well-developed hatchlings (Hua, Nande, Doubleday, & Gillanders, 2023). Following capture, octopuses were transported in individual, 12 L aerated buckets of local seawater (15 °C) kept in insulated bags. Dens in the form of sectioned PVC pipes (65 mm diameter, 20 cm length) were provided for each octopus during transport. Octopuses were transported to the South Australian Research and Development Institute (SARDI) in Adelaide, where all experiments were conducted in a controlled environment room. All octopuses remained in their respective dens during transport and were transferred in their dens from the buckets into separate glass tanks (50 cm × 2..., , # Weights of hatchlings across different temperature treatments

[https://doi.org/10.5061/dryad.ghx3ffbwq](https://doi.org/10.5061/dryad.ghx3ffbwq)

Research Article: Projected ocean temperatures impair key proteins used in vision of octopus hatchlings (Journal: Global Change Biology)

This dataset contains raw weights (mg) of hatchlings across the control (19°C), current (22°C), and future (25°C) temperatures in CSV as well as the R script used to generate the boxplots (Fig. 4 in manuscript).

## Description of the data and file structure

**CSV Raw Data of Hatchling Weights:**

**Mother ID**: refers to the octopus brood/mother which also contains the temperature e.g. 19B refers to the octopus mother B from 19°C.

**Temperature**: refers to the thermal condition that each octopus individual was exposed to i.e. 19°C, 22°C, or 25°C.

**HatchlingID**: refers to the unique identifier of each hatchling containing the octopus brood/mother and its unique identifier

**UsedForProteomics**: ref...