Inactivation of M. tuberculosis H37Rv in sputum by using the biosafe protocol.
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1H37Rv cells were suspended in sputum samples at concentrations of approximately 1×107 CFU/mL. After treatment, 0.1 mL aliquots were plated on triplicate Middlebrook 7H10 plates with OADC supplement. Numbers shown are total counts for the three plates after 33 days of incubation at 37°C.2Because untreated sputum samples rapidly overgrew plates due to the natural flora of sputum, no-treatment controls consisted of M. tuberculosis cells suspended in PBS.3TNTC, too numerous to count.
创建时间：
2015-12-02
