16S rDNA sequencing from stool of mice with different iron diets

Iron deficiency is one of the most common nutritional deficiencies worldwide and is often treated with oral iron supplementation. However, commonly used supplements based on ferrous iron salts are associated with gastrointestinal side effects and unfavorable changes to the microbiome. Sucrosomial iron is a novel iron formulation that is effective at treating iron deficiency, but with fewer gastrointestinal side effects. However, its effect on the microbiome has not been examined previously. Thus, we have treated mice for two weeks with diets containing either Sucrosomial iron or ferrous sulfate as the sole iron source and examined the effect on the microbial communities using 16S Microbial Profiling of DNA extracted from feces collected both prior to and following dietary treatment.