Blood clinical chemistry analysis for each mouse.

The mice were randomly divided into three groups and treated with either Vehicle or two concentrations of PPM201 (6 or 20 mg/kg body weight). The response to the “therapeutic dose”, 6 mg/kg, was found to vary widely for ALT (alanine aminotransferase), AST (aspartate aminotransferase), LDH (lactate dehydrogenase) and CK (creatine kinase). AST is raised in PPM201 treated animals, with mouse E (6 mg/kg) seeming to be especially raised; AST is known to be variable between animals, but mouse E also shows a higher level of ALT, indicating that there may be a shared mechanism for the two enzymes. Creatinine is decreased in liver and possibly kidney disease; the contrasts observed here are inconclusive. BUN (Blood, Urea and Nitrogen) is raised in kidney disease; results are again inconclusive. Following cardiac infarction LDH is increased after 12 hours, possibly also caused by liver toxicity; mouse E is markedly lower than the other PPM201 treated animals and it may be that its heart muscle profile might be more similar to the untreated mice. CK is, like LDH, increased in myocardial infarction and this supports the LDH findings for mouse E.