Gene expression changes in mouse fibroblasts (3T3) after sunitinib resistance and acute and chronic withdrawal

Transcriptome analysis was used to identify changes in mouse fibroblasts (3T3) resistant to sunitinib (SuR) and after short and long term sunitinib withdrawal in vitro (ST-W for 48 hours and LT-W for more than 2 months, respectively). Mouse fibroblasts (3T3) resistant to sunitinib were derived in vitro after incremental increases of sunitinib from 250nM to 5μM. Resistant cells were maintained in vitro with 5μM sunitinib for more than 2 months.