Mutational effects on the catalytic activity (<i>k</i><sub>cat</sub>/<i>K</i><sub>m</sub> ratio) and thermal stability (Δ<i>T</i><sub>m</sub>) of Sfβgly proteins.
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Only mutational effects higher than a 4-fold change in the kcat/Km ratio (0.25>kcat/Km ratio >4) were considered significant for the enzymatic activity. Mutational effects on the thermal stability were considered relevant only for ΔTm>2.5 K. –, no activity; nd, not determined; wt, wild-type.
创建时间:
2014-05-07



