ß-sitosterol-mediated cardioprotection: a transcriptomic perspective

ß-sitosterol has been implicated in mitigating cardiovascular diseases, yet its role in MI remains underexplored. This study investigates the therapeutic potential and underlying mechanisms of ß-sitosterol in MI using both in vivo and in vitro models.Transcriptome sequencing combined with molecular docking and protein validation assays revealed that ß-SITO exerts its beneficial effects primarily through the regulation of inflammatory and necroptotic signaling pathways, particularly via modulation of NF-?B, NLRP3 inflammasome, and RIPK3/MLKL-mediated necroptosis. Overall design: RNA-seq analysis of HO-induced NMCMs treated with either vehicle or ß-SITO.